Chromosomal locations of the ribosomal DNA genes in shortleaf pine (2007)Islam-Faridi, N., Majid, M. A., & Nelson, C. D. (2007). Chromosomal locations of the ribosomal DNA genes in shortleaf pine. Shortleaf pine restoration and ecology in the Ozarks: proceedings of a symposium; 2006 November 7-9; Springfield, MO. Northern Research Station, General Technical Report NRS-P-15. Retrieved from https://www.nrs.fs.fed.us/pubs/gtr/gtr_p-15%20papers/14islam-faridi-p-15.pdf A reference karyotype (i.e., chromosome-specific description of a species' chromosomal complement) is a pre-requisite for advanced genetic and genomic studies. The Southern Institute of Forest Genetics has initiated a project to develop reference karyotypes for each of the major southern U.S. pine species, including shortleaf pine, using AT-rich chromosomal banding and fluorescent in situ hybridization (FISH). About half of the project has been completed to date, including the development of karyotypes for loblolly pine and slash pine, with the remaining experiments being directed towards shortleaf pine and longleaf pine. Preliminary FISH results for rDNA genes in shortleaf pine show that there are seven major, and as many as eight medium-to-minor centromeric 18S-28S rDNA sites. In addition, one major and one minor 5S rDNA sites were observed and most of the chromosomes showed AT-rich bands. A complete shortleaf pine karyotype is being developed for comparison with other pine and conifer species
https://www.nrs.fs.fed.us/pubs/gtr/gtr_p-15%20papers/14islam-faridi-p-15.pdf |
